Simulation Technology for Life Sciences



Software and Methods for Computer-Aided Bioprocess Development


GoSilico's ChromX chromatography simulation software allows your chromatography lab experiments to be brought to the computer. Initialize a computer model of your column with few lab experiments and simulate all further lab experiments in-silico.



A Versatile Simulation Software for Preparative Liquid Chromatography Processes

Save Time and Money

Skip up to 95% of your lab experiments to achieve process optimization and characterization.


Improve Quality, Reduce Risks

Perform in-silico process optimization, robustness studies, and worst-case analyses.

Utilize Quality-by-Design

Gain a mechanistic understanding of your process and fulfill regulatory expectations.


Meet the Team Behind GoSilico

Dr. Tobias Hahn

Dr. Tobias Hahn

Chief Executive Officer

During his PhD in the field of biotechnology, Tobias utilized his background in applied mathematics and software development to create the chromatography simulation software ChromX. As a co-founder of GoSilico, he’s responsible for product development and consultancy. Besides facilitating chromatography simulation, he’s passionate about outdoor activities and his little family.

Dr. Thiemo Huuk

Dr. Thiemo Huuk

Chief Executive Officer

Thiemo did a PhD in biotechnology, where he gained a profound background and expertise in the downstream process development of biopharmaceuticals. As one of the co-founders of GoSilico, he’s responsible for initiating and managing customer partnerships. Beyond shaping a simulation-driven future for biopharma process development, his biggest passions are sailing and yoga.

Dr. Teresa Baumann

Dr. Teresa Baumann

Chief Operating Officer

In her PhD in applied mathematics, Teresa used to simulate weather and climate phenomena. Since her former math colleague Tobias invited her to join GoSilico, she switched to simulating chromatography. As a co-founder of GoSilico, she’s responsible for corporate functions and communication. Whenever she’s not going silico, she likes running, biking and contemporary Jazz.

Prof. Dr. Jürgen Hubbuch

Prof. Dr. Jürgen Hubbuch

Scientific Advisor

As a professor in biopharmaceutical process engineering at Karlsruhe Institute of Technology, he has a vast overview over novel technologies and trends in the purification of bioproducts. At GoSilico, he brings in his industrial insight, expertise and vision as a technology scout. Besides being a university professor and a co-founder of GoSilico, he spends most of his free time raising his three children.

Alexander Gutzler

Alexander Gutzler

Student Assistant

After finishing his Bachelor in bioengineering, Alex spent a year with internships in pharmaceutical downstream processing. Not only has he worked with some of the best in the industry, but he has also gained valuable insight into ChromX from a user’s perspective. Apart from enabling user-friendly chromatography simulation, he enjoys all sports involving boards and water in a liquid or solid state.

Till Briskot

Till Briskot

Research Engineer

After finishing his Master in bioengineering at Karlsruhe Institute of Technology, Till worked for Roche Diagnostics GmbH in Penzberg and gained profound experience in process modeling. As a research engineer at GoSilico, he uses his practical experience to support product development. While he spends most of his work live in silico, he uses his spare time for triathlon training.

Anna Schubert

Anna Schubert

Team Assistant

Before joining GoSilico, Anna graduated in business administration, worked as a project manager and eventually founded her own start-up. At GoSilico, Anna is responsible for administration and support in finance management, HR and marketing. As a passionate and certified graphic designer, Anna loves to work on creative projects such as photography and web design.

Tarun Mamachan Sajitha

Tarun Mamachan Sajitha

Software Engineer

After completing Bachelor of Technology from National Institute of Technology Calicut in India, Tarun enrolled for Master of Science program in Computational Engineering at Ruhr University Bochum where he specialized in mathematical modelling and finite element simulations. Along with his master studies he worked as Scientific Assistant in Biomechanics team in Lehrstuhl für Produktentwicklung and also as Werk Student in Product Development team at HUF Gmbh. At GoSilico, Tarun supports the software development and software testing team. Besides his interest in software and simulation, he is a nature lover and a multi-instrumentalist. In his free time, he likes to play guitar or piano or do some gardening.

Thomas Becker

Thomas Becker

Master's Student

During his Bachelor's and Master's studies in bioengineering at Karlsruhe Institute of Technology and an internship at Evonik Industries AG, Thomas gained comprehensive knowledge in particle engineering and biopharmaceutical processing. Working on his Master's thesis at GoSilico, he is part of the research team to enhance our simulation technology. Aside from contributing to biopharmaceutical processing research, he likes to ride his motorcycle and to tinker around with electronic and non-electronic stuff.

Nora Geng

Nora Geng

Project Engineer

Completing her Bachelor's and Master's degree in biological engineering at the Karlsruhe Institute of Technology (KIT), Nora gained a lot of knowledge of biopharma downstream processing. As a part of the GoSilico service team she participates in customer project support and trainings. In her free time she is very much in love with reading and sports, especially volleyball and Esdo.



Interested in working with us?

GoSilico is an aspiring young company located in Karlsruhe, Germany, that serves customers worldwide. We are continuously looking for new team members, motivated to foster a cutting edge simulation technology and to enable the next generation of pharmaceutical bioprocess development.

Open Positions

Account Manager

Project Engineer

Sales Manager

Software Engineer

Please send your application to


Stay Up to Date About the Latest News About GoSilico

Happy Birthday, GoSilico!

Happy Birthday, GoSilico!

Karlsruhe, 9th of February 2019. It has been more than three years, since we launched GoSilico. Time to look back – and ahead. GoSilico’s c ...

Chromatography Modeling Days 2019

Chromatography Modeling Days 2019

GoSilico is very pleased to invite you to the first Chromatography Modeling Days (CMD19), on 27th-29th of May in Mainz, Germany. CMD19 offer ...

New Year, New Conference Season

New Year, New Conference Season

Karlsruhe, 23rd of January. The new year just started and we’re highly motivated planning the upcoming conference season. During the next mo ...

Success Stories

Gain Insight into Chromatography Modeling and Experience Success Stories Facilitated by ChromX


  • All
  • Model Development
  • Process Optimization
  • Process Characterization
  • Facility Fit and Scale Up
  • Model Development for Hydrophobic Interaction Chromatography

    Model Development
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  • Model Development for Polymer Fibers as Stationary Phases

    Model Development
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  • Model-Based Process Development for Virus-Like Particles

    Process Optimization
    Permalink Image
  • Optimal Experimental Design for the Determination of Isotherm Parameters

    Model Development
    Permalink Image
  • Optimization of an Antibody Polishing Step

    Process Optimization
    Permalink Image
  • High-Throughput Micro-Scale Cultivations and Chromatography Modeling

    Process Characterization
    Permalink Image
  • Modeling of Complex Protein Elution Behavior

    Model Development
    Permalink Image
  • Process Optimization for an Industrial Cation Exchange Step

    Process Optimization
    Permalink Image
  • Process Characterization for Troubleshooting

    Process Characterization
    Permalink Image
  • Adsorber Quantification in Batch and Column Chromatography

    Facility Fit and Scale Up
    Permalink Image


  • Over the past few decades, science has laid the foundation for computer-guided process development. We've done our job. Now it's up to you to take advantage of the scientific achievements and go in silico.

    Prof. Dr. Jürgen Hubbuch
    Prof. Dr. Jürgen Hubbuch, KIT




Mechanistic Extension of a Hydrophobic Interaction Chromatography Model to Account for pH Changes and Mixed-Mode Binding.
Tobias Hahn, Gang Wang, Theresa Läufer, Maria Casals Peralvarez, Thiemo Huuk, Jürgen Hubbuch
Poster presented at: Recovery of Biological Products XVIII; 10/2018; Asheville, NC, USA. Download.

Mechanistic Modeling as a Tool to Streamline Downstream Process Development, Scale-up, and Characterization.
Thiemo Huuk, Tobias Hahn, Jürgen Hubbuch
Poster presented at: BioProcess International (BPI) Conference & Exhibition; 09/2018; Boston, MA, USA. Download.

Next generation process development: Stop Experimenting. GoSilico.
Thiemo Huuk, Tobias Hahn, Teresa Beck, Jürgen Hubbuch
Poster presented at: 12th European Symposium on Biochemical Engineering Sciences (ESBES) ; 09/2018; Lisbon, Portugal. Download.
Poster presented at: 31st International Symposium on Preparative and Process Chromatography; 07/2018; Baltimore, Maryland, USA. Download.
Poster presented at: BioProcess International European Summit; 04/2018; Amsterdam, The Netherlands. Download.

Modified Batch Isotherm Determination Method for Mechanistic Model Calibration
Tobias Hahn, Thiemo Huuk, Juergen Hubbuch (KIT, Karlsruhe)
Oral presentation at: ACS Spring Meeting;03/2018; New Orleans, USA. View slides online.
Extended report of the Fourth HTPD conference in Toledo, Spain, October 2017, 6-9. Download.

Scalability of Mechanistic Models for Ion Exchange Chromatography under High Load Conditions
Thiemo Huuk, Tobias Hahn, Jan Griesbach (F. Hoffmann-La Roche Ltd, Basel), Stefan Hepbildikler (Roche Diagnostics GmbH, Penzberg), Jürgen Hubbuch (KIT, Karlsruhe)
Poster presented at: The Fourth International Conference devoted to high-throughput process development (HTPD); 10/2017; Toledo, Spain. Download.
Extended report of the Fourth HTPD conference in Toledo, Spain, October 2017, 10-12. Download.

When DoE fails: Mechanistic modeling for chromatographic manufacturability
Tobias Hahn, Thiemo Huuk, Teresa Beck, Jürgen Hubbuch
Poster presented at: 30th International Symposium on Preparative and Process Chromatography (PREP); 07/2017; Philadelphia. Download.
Poster presented at: 253rd ACS National Meeting; 04/2017; San Francsisco. Download.

Estimation of adsorption isotherm and mass transfer parameters in protein chromatography using artificial neural networks
Gang Wang, Till Briskot, Tobias Hahn, Pascal Baumann, Jürgen Hubbuch
Journal of Chromatography A 03/2017; 1487:211–217. DOI:10.1016/j.chroma.2017.01.068

Modeling of complex antibody elution behavior under high protein load densities in ion exchange chromatography using an asymmetric activity coefficient
Thiemo C. Huuk, Tobias Hahn, Katharina Doninger, Jan Griesbach, Stefan Hepbildikler, Jürgen Hubbuch
Biotechnology Journal 03/2017, 1600336. DOI:10.1002/biot.201600336

A mechanistic model of ion-exchange chromatography on polymer fiber stationary phases
Johannes Winderl, Tobias Hahn, Jürgen Hubbuch
Journal of Chromatography A 10/2016; 1475:18–30. DOI:10.1016/j.chroma.2016.10.057

Water on Hydrophobic Surfaces: Mechanistic Modeling of Hydrophobic Interaction Chromatography
Gang Wang, Tobias Hahn, Jürgen Hubbuch
Journal of Chromatography A 07/2016; 1465:71–78. DOI:10.1016/j.chroma.2016.07.085

In-Silico Process Development: Success Stories
Teresa Beck, Tobias Hahn, Thiemo Huuk, Jürgen Hubbuch
Poster presented at: 29th International Symposium on Preparative and Process Chromatography; 07/2016; Philadelphia, Pennsylvania, USA. Download.

Model-based conversion of a single-column batch process to 3- and 4-column periodic counter-current chromatography
Tobias Hahn, Thiemo Huuk, Fabian Görlich, Gang Wang, Jürgen Hubbuch
Poster presented at: Recovery of Biological Products XVII; 06/2016; Bermuda. Download.
Poster presented at: 29th International Symposium on Preparative and Process Chromatography; 07/2016; Philadelphia, Pennsylvania, USA. Download.

No use for high-throughput batch isotherms in modeling?
Thiemo Huuk, Tobias Hahn, Gang Wang, Jürgen Hubbuch
Poster presented at: Recovery of Biological Products XVII; 06/2016; Bermuda. Download.

A versatile noninvasive method for adsorber quantification in batch and column chromatography based on the ionic capacity
Thiemo Huuk, Tobias Hahn, Jürgen Hubbuch
Biotechnology Progress 05/2016; 32(3):666–677. DOI:10.1002/btpr.2228

Application of spectral deconvolution and inverse mechanistic modelling as a tool for root cause investigation in protein chromatography
Nina Brestrich, Tobias Hahn, Jürgen Hubbuch
Journal of Chromatography A 03/2016; 1437:158–167. DOI:10.1016/j.chroma.2016.02.011

Modeling and simulation of anion-exchange membrane chromatography for purification of Sf9 insect cell-derived virus-like particles
Christopher Ladd Effio, Tobias Hahn, Julia Seiler, Stefan Oelmeier, Iris Asen, Christine Silberer, Louis Villain, Jürgen Hubbuch
Journal of Chromatography A 01/2016; 1429:142-154. DOI:10.1016/j.chroma.2015.12.006

Insights into chromatographic separation using core-shell metal-organic frameworks: size exclusion and polarity effects
Weiwei Qin, Martin E. Silvestre, Frank Kirschhöfer, Gerald Brenner-Weiss, Matthias Franzreb
Journal of Chromatography A 09/2015; 1411:77–83. DOI:10.1016/j.chroma.2015.07.120

Simulating and Optimizing Preparative Protein Chromatography with ChromX
Tobias Hahn, Thiemo Huuk, Vincent Heuveline, Jürgen Hubbuch
Journal of Chemical Education 09/2015; 92:1497–1502. DOI:10.1021/ed500854a

High-throughput micro-scale cultivations and chromatography modeling: Powerful tools for integrated process development
Pascal Baumann, Tobias Hahn, Jürgen Hubbuch
Biotechnology and Bioengineering 10/2015; 112:2123–2133. DOI:10.1002/bit.25630

Calibration-free inverse modeling of ion-exchange chromatography in industrial antibody purification
Tobias Hahn, Thiemo Huuk, Anna Osberghaus, Katharina Doninger, Susanne Nath, Stefan Hepbildikler, Vincent Heuveline, Jürgen Hubbuch
Engineering in Life Sciences 05/2015; 16:107–113. DOI:10.1002/elsc.201400248

UV absorption-based inverse modelling of protein chromatography
Tobias Hahn, Pascal Baumann, Thiemo Huuk, Vincent Heuveline, Jürgen Hubbuch
Engineering in Life Sciences 04/2015; 16:99–106. DOI:10.1002/elsc.201400247

Model-based integrated optimization and evaluation of a multi-step ion exchange chromatography
Thiemo C. Huuk, Tobias Hahn, Anna Osberghaus, Jürgen Hubbuch
Separation and Purification Technology 11/2014; 136:207–222. DOI:10.1016/j.seppur.2014.09.012

Adjoint-based Estimation and Optimization for Column Liquid Chromatography Models
Tobias Hahn, Anja Sommer, Anna Osberghaus, Vincent Heuveline, Jürgen Hubbuch
Computers & Chemical Engineering 05/2014; 64. DOI:10.1016/j.compchemeng.2014.01.013

A high-throughput 2D-analytical technique to obtain single protein parameters from complex cell lysates for in silico process development of ion exchange chromatography
Frieder Kröner, Dennis Elsäßer, Jürgen Hubbuch
Journal of Chromatography A 11/2013; 1318. DOI:10.1016/j.chroma.2013.09.043

ChromX - A Powerful and User-Friendly Software Package for Modeling Liquid Chromatography Processes
Tobias Hahn, Vincent Heuveline, Jürgen Hubbuch
Chemie Ingenieur Technik 08/12; 84(8). DOI:10.1002/cite.201250071

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