ChromX

A versatile simulation software for preparative liquid chromatography processes.

Save Time and Money

Skip up to 95% of your lab experiments to achieve process optimization and characterization.

 

Improve Quality, Reduce Risks

Perform in-silico process optimization, robustness studies, and worst-case analyses.

Utilize Quality-by-Design

Gain a mechanistic understanding of your process and fulfill regulatory expectations.

Why ChromX?

Discover the benefits of the world's leading downstream chromatography simulation software.

 

ChromX is a versatile simulation software application for preparative liquid chromatography processes, that simplifies model-based process development. Import few experimental UV signals for calibrating the model and simulate all further lab experiments in-silico.

 

 

Highly Customizable

The modular architecture of ChromX allows the simulation of any kind of single and multi-column process in any relevant mode of chromatography. Model adsorption by utilizing one of the built-in state-of-the-art isotherm equations or add your own, customized adsorption model equation.

 

Excellent Simulation Framework

Thanks to the robust and versatile simulation backend of ChromX, you’ll be able to get your chromatography model simulation running within minutes – and without all the tedious fuss of implementing and configuring a numerical equation solver yourself. Since ChromX utilizes high-performance computing methods, we can guarantee you an outstanding simulation speed.

 

Simple & Powerful User Interface

ChromX intuitive user interface provides easy access to the control features of your chromatography process, such as dimensions, flow rates, buffer concentrations or sample injection. Control parameters can then be optimized in respect to your own objectives. Unknown parameters can be estimated from experimental UV signals, which can easily be imported in the software.

 

Success Stories

Gain insight into chromatography modeling and experience ChromX' success stories.

 

  • All
  • Facility Fit and Scale Up
  • Process Characterization
  • Process Optimization
  • Model Development
  • Adsorber Quantification in Batch and Column Chromatography

    Facility Fit and Scale Up
    Permalink Image
  • Process Characterization for Troubleshooting

    Process Characterization
    Permalink Image
  • Process Optimization for an Industrial Cation Exchange Step

    Process Optimization
    Permalink Image
  • Modeling of Complex Protein Elution Behavior

    Model Development
    Permalink Image
  • High-Throughput Micro-Scale Cultivations and Chromatography Modeling

    Process Characterization
    Permalink Image
  • Optimization of an Antibody Polishing Step

    Process Optimization
    Permalink Image
  • Optimal Experimental Design for the Determination of Isotherm Parameters

    Model Development
    Permalink Image
  • Model-Based Process Development for Virus-Like Particles

    Process Optimization
    Permalink Image
  • Model Development for Polymer Fibers as Stationary Phases

    Model Development
    Permalink Image
  • Model Development for Hydrophobic Interaction Chromatography

    Model Development
    Permalink Image

 

Scientific References

Learn from academic and industrial case studies.

Application of mechanistic modeling to speed up purification process development of novel formats: A virus-like particle case study
Tobias Hahn, Maria Casals, Thiemo Huuk, Jens Wölfel, Christian Niehus, Nikola Strempel
Poster presented at: 257th ACS National Meeting; 04/2019; Orlando, FL, USA. Download.

Modeling of ion-exchange chromatography: Are we making the right assumptions?
Till Briskot, Tobias Hahn, Thiemo Huuk, Jürgen Hubbuch
Poster presented at: 257th ACS National Meeting; 04/2019; Orlando, FL, USA. Download.

Mechanistic Modeling as a Tool to Streamline Downstream Process Development, Scale-Up, and Characterization
Thiemo Huuk, Tobias Hahn, Jürgen Hubbuch
Poster presented at: BioProcess International Asia; 02/2019; Tokyo, Japan. Download.
Poster presented at: BioProcess International West; 02/2019; Santa Clara, USA. Download.
Poster presented at: BioProcess International Europe; 04/2019; Vienna, Austria. Download.

Mechanistic Extension of a Hydrophobic Interaction Chromatography Model to Account for pH Changes and Mixed-Mode Binding.
Tobias Hahn, Gang Wang, Theresa Läufer, Maria Casals Peralvarez, Thiemo Huuk, Jürgen Hubbuch
Poster presented at: Recovery of Biological Products XVIII; 10/2018; Asheville, NC, USA. Download.

Mechanistic Modeling as a Tool to Streamline Downstream Process Development, Scale-up, and Characterization.
Thiemo Huuk, Tobias Hahn, Jürgen Hubbuch
Poster presented at: BioProcess International (BPI) Conference & Exhibition; 09/2018; Boston, MA, USA. Download.

Next generation process development: Stop Experimenting. GoSilico.
Thiemo Huuk, Tobias Hahn, Teresa Beck, Jürgen Hubbuch
Poster presented at: 12th European Symposium on Biochemical Engineering Sciences (ESBES) ; 09/2018; Lisbon, Portugal. Download.
Poster presented at: 31st International Symposium on Preparative and Process Chromatography; 07/2018; Baltimore, Maryland, USA. Download.
Poster presented at: BioProcess International European Summit; 04/2018; Amsterdam, The Netherlands. Download.

Modified Batch Isotherm Determination Method for Mechanistic Model Calibration
Tobias Hahn, Thiemo Huuk, Juergen Hubbuch (KIT, Karlsruhe)
Oral presentation at: ACS Spring Meeting;03/2018; New Orleans, USA. View slides online.
Extended report of the Fourth HTPD conference in Toledo, Spain, October 2017, 6-9. Download.

Scalability of Mechanistic Models for Ion Exchange Chromatography under High Load Conditions
Thiemo Huuk, Tobias Hahn, Jan Griesbach (F. Hoffmann-La Roche Ltd, Basel), Stefan Hepbildikler (Roche Diagnostics GmbH, Penzberg), Jürgen Hubbuch (KIT, Karlsruhe)
Poster presented at: The Fourth International Conference devoted to high-throughput process development (HTPD); 10/2017; Toledo, Spain. Download.
Extended report of the Fourth HTPD conference in Toledo, Spain, October 2017, 10-12. Download.

When DoE fails: Mechanistic modeling for chromatographic manufacturability
Tobias Hahn, Thiemo Huuk, Teresa Beck, Jürgen Hubbuch
Poster presented at: 30th International Symposium on Preparative and Process Chromatography (PREP); 07/2017; Philadelphia. Download.
Poster presented at: 253rd ACS National Meeting; 04/2017; San Francsisco. Download.

Estimation of adsorption isotherm and mass transfer parameters in protein chromatography using artificial neural networks
Gang Wang, Till Briskot, Tobias Hahn, Pascal Baumann, Jürgen Hubbuch
Journal of Chromatography A 03/2017; 1487:211–217. DOI:10.1016/j.chroma.2017.01.068

Modeling of complex antibody elution behavior under high protein load densities in ion exchange chromatography using an asymmetric activity coefficient
Thiemo C. Huuk, Tobias Hahn, Katharina Doninger, Jan Griesbach, Stefan Hepbildikler, Jürgen Hubbuch
Biotechnology Journal 03/2017, 1600336. DOI:10.1002/biot.201600336

A mechanistic model of ion-exchange chromatography on polymer fiber stationary phases
Johannes Winderl, Tobias Hahn, Jürgen Hubbuch
Journal of Chromatography A 10/2016; 1475:18–30. DOI:10.1016/j.chroma.2016.10.057

Water on Hydrophobic Surfaces: Mechanistic Modeling of Hydrophobic Interaction Chromatography
Gang Wang, Tobias Hahn, Jürgen Hubbuch
Journal of Chromatography A 07/2016; 1465:71–78. DOI:10.1016/j.chroma.2016.07.085

In-Silico Process Development: Success Stories
Teresa Beck, Tobias Hahn, Thiemo Huuk, Jürgen Hubbuch
Poster presented at: 29th International Symposium on Preparative and Process Chromatography; 07/2016; Philadelphia, Pennsylvania, USA. Download.

Model-based conversion of a single-column batch process to 3- and 4-column periodic counter-current chromatography
Tobias Hahn, Thiemo Huuk, Fabian Görlich, Gang Wang, Jürgen Hubbuch
Poster presented at: Recovery of Biological Products XVII; 06/2016; Bermuda. Download.
Poster presented at: 29th International Symposium on Preparative and Process Chromatography; 07/2016; Philadelphia, Pennsylvania, USA. Download.

No use for high-throughput batch isotherms in modeling?
Thiemo Huuk, Tobias Hahn, Gang Wang, Jürgen Hubbuch
Poster presented at: Recovery of Biological Products XVII; 06/2016; Bermuda. Download.

A versatile noninvasive method for adsorber quantification in batch and column chromatography based on the ionic capacity
Thiemo Huuk, Tobias Hahn, Jürgen Hubbuch
Biotechnology Progress 05/2016; 32(3):666–677. DOI:10.1002/btpr.2228

Application of spectral deconvolution and inverse mechanistic modelling as a tool for root cause investigation in protein chromatography
Nina Brestrich, Tobias Hahn, Jürgen Hubbuch
Journal of Chromatography A 03/2016; 1437:158–167. DOI:10.1016/j.chroma.2016.02.011

Modeling and simulation of anion-exchange membrane chromatography for purification of Sf9 insect cell-derived virus-like particles
Christopher Ladd Effio, Tobias Hahn, Julia Seiler, Stefan Oelmeier, Iris Asen, Christine Silberer, Louis Villain, Jürgen Hubbuch
Journal of Chromatography A 01/2016; 1429:142-154. DOI:10.1016/j.chroma.2015.12.006

Insights into chromatographic separation using core-shell metal-organic frameworks: size exclusion and polarity effects
Weiwei Qin, Martin E. Silvestre, Frank Kirschhöfer, Gerald Brenner-Weiss, Matthias Franzreb
Journal of Chromatography A 09/2015; 1411:77–83. DOI:10.1016/j.chroma.2015.07.120

Simulating and Optimizing Preparative Protein Chromatography with ChromX
Tobias Hahn, Thiemo Huuk, Vincent Heuveline, Jürgen Hubbuch
Journal of Chemical Education 09/2015; 92:1497–1502. DOI:10.1021/ed500854a

High-throughput micro-scale cultivations and chromatography modeling: Powerful tools for integrated process development
Pascal Baumann, Tobias Hahn, Jürgen Hubbuch
Biotechnology and Bioengineering 10/2015; 112:2123–2133. DOI:10.1002/bit.25630

Calibration-free inverse modeling of ion-exchange chromatography in industrial antibody purification
Tobias Hahn, Thiemo Huuk, Anna Osberghaus, Katharina Doninger, Susanne Nath, Stefan Hepbildikler, Vincent Heuveline, Jürgen Hubbuch
Engineering in Life Sciences 05/2015; 16:107–113. DOI:10.1002/elsc.201400248

UV absorption-based inverse modelling of protein chromatography
Tobias Hahn, Pascal Baumann, Thiemo Huuk, Vincent Heuveline, Jürgen Hubbuch
Engineering in Life Sciences 04/2015; 16:99–106. DOI:10.1002/elsc.201400247

Model-based integrated optimization and evaluation of a multi-step ion exchange chromatography
Thiemo C. Huuk, Tobias Hahn, Anna Osberghaus, Jürgen Hubbuch
Separation and Purification Technology 11/2014; 136:207–222. DOI:10.1016/j.seppur.2014.09.012

Adjoint-based Estimation and Optimization for Column Liquid Chromatography Models
Tobias Hahn, Anja Sommer, Anna Osberghaus, Vincent Heuveline, Jürgen Hubbuch
Computers & Chemical Engineering 05/2014; 64. DOI:10.1016/j.compchemeng.2014.01.013

A high-throughput 2D-analytical technique to obtain single protein parameters from complex cell lysates for in silico process development of ion exchange chromatography
Frieder Kröner, Dennis Elsäßer, Jürgen Hubbuch
Journal of Chromatography A 11/2013; 1318. DOI:10.1016/j.chroma.2013.09.043

ChromX - A Powerful and User-Friendly Software Package for Modeling Liquid Chromatography Processes
Tobias Hahn, Vincent Heuveline, Jürgen Hubbuch
Chemie Ingenieur Technik 08/12; 84(8). DOI:10.1002/cite.201250071

Try ChromX Now!

Get your ChromX test license today.

Our Office

GoSilico GmbH
Kaiserstr. 183
76133 Karlsruhe

Contact Info

Phone: +49 721 754 016 90
Email: info@gosilico.com
Web: www.gosilico.com

 

 

If you are interested in the commercial use of ChromX, please fill in the following contact form. We will get in contact with you and will inform you about special features and pricing. Upon request, we can offer you a free 30-day trial version.

I have read and understood GoSilico's privacy policy and consent to the collection, use and disclosure of my personal data by GoSilico for the purpose of contacting me.
I herewith allow GoSilico to contact me via the following channels:



Email Newsletter

As an academic, you can use ChromX for free. Please leave your contact information here to receive a verification e-mail containing a download link. You can download and test ChromX for 30 days. In the meantime, we will validate that your credentials belong to a scientific institution and send you a separate license key that enables you to use ChromX beyond the 30 day trial period.

I have read and understood GoSilico's privacy policy and consent to the collection, use and disclosure of my personal data by GoSilico for the purpose of contacting me.
I herewith allow GoSilico to contact me via the following channels:



Email Newsletter