GoSilico publishes joint work with Roche
Karlsruhe, 20th of November. GoSilico is happy to announce the publication of joint work with Roche on the scalability of mechanistic models for ion exchange chromatography under high load conditions.
Within this joint case study, ChromX was successfully applied to model an intermediate purification step of a monoclonal antibody. The key challenge was the combination of high protein load density and low salt concentration that hindered modeling attempts basing on common models for ion exchange chromatography. Instead, an extended model was successfully applied to match the observed unusual peak shape.
The model was calibrated from 1 mL and 0.6 mL measurements and successfully utilized for an in-silico scale-up to 16 mL scale. The results gave clear evidence that the adsorption behavior is independent of the scale of a column, even for complex adsorption behavior. In fact, only the fluid dynamics outside the pore system change with the scale.
The poster entitled “Scalability of mechanistic models for ion exchange chromatography under high load conditions” had previously been presented on HTPD conference in October this year and is available here.