GoSilico publishes joint work with Roche
Karlsruhe, November 20th. GoSilico is happy to announce the publication of joint project with Roche on the scalability of mechanistic models for ion exchange chromatography under high load conditions.
Within this case study, ChromX was successfully applied to model an intermediate purification step of a monoclonal antibody. The key challenge was the combination of high protein load density and low salt concentration which hindered modeling attempts based on common models for ion exchange chromatography. Instead, an extended model was successfully applied to match the unusual peak shape observed.
The model was calibrated from 1 mL and 0.6 mL measurements and successfully utilized for an in-silico scale-up to 16 mL. The results provided clear evidence that the adsorption behavior is independent of the scale of a column, even for complex adsorption behavior. In fact, only the fluid dynamics outside the pore system change with the scale.
The poster with the title “Scalability of mechanistic models for ion exchange chromatography under high load conditions” had previously been presented at HTPD conference in October this year and is available here.