Scientific References

When DoE fails: Mechanistic modeling for chromatographic manufacturability
Tobias Hahn, Thiemo Huuk, Teresa Beck, Jürgen Hubbuch
Poster presented at: 253rd ACS National Meeting; 04/2017; San Francsisco. Download.

Estimation of adsorption isotherm and mass transfer parameters in protein chromatography using artificial neural networks
Gang Wang, Till Briskot, Tobias Hahn, Pascal Baumann, Jürgen Hubbuch
Journal of Chromatography A 03/2017; 1487:211–217. DOI:10.1016/j.chroma.2017.01.068

Modeling of complex antibody elution behavior under high protein load densities in ion exchange chromatography using an asymmetric activity coefficient
Thiemo C. Huuk, Tobias Hahn, Katharina Doninger, Jan Griesbach, Stefan Hepbildikler, Jürgen Hubbuch
Biotechnology Journal 03/2017, 1600336. DOI:10.1002/biot.201600336

A mechanistic model of ion-exchange chromatography on polymer fiber stationary phases
Johannes Winderl, Tobias Hahn, Jürgen Hubbuch
Journal of Chromatography A 10/2016; 1475:18–30. DOI:10.1016/j.chroma.2016.10.057

Water on Hydrophobic Surfaces: Mechanistic Modeling of Hydrophobic Interaction Chromatography
Gang Wang, Tobias Hahn, Jürgen Hubbuch
Journal of Chromatography A 07/2016; 1465:71–78. DOI:10.1016/j.chroma.2016.07.085

In-Silico Process Development: Success Stories
Teresa Beck, Tobias Hahn, Thiemo Huuk, Jürgen Hubbuch
Poster presented at: 29th International Symposium on Preparative and Process Chromatography; 07/2016; Philadelphia, Pennsylvania, USA. Download.

Model-based conversion of a single-column batch process to 3- and 4-column periodic counter-current chromatography
Tobias Hahn, Thiemo Huuk, Fabian Görlich, Gang Wang, Jürgen Hubbuch
Poster presented at: Recovery of Biological Products XVII; 06/2016; Bermuda. Download.
Poster presented at: 29th International Symposium on Preparative and Process Chromatography; 07/2016; Philadelphia, Pennsylvania, USA. Download.

No use for high-throughput batch isotherms in modeling?
Thiemo Huuk, Tobias Hahn, Gang Wang, Jürgen Hubbuch
Poster presented at: Recovery of Biological Products XVII; 06/2016; Bermuda. Download.

A versatile noninvasive method for adsorber quantification in batch and column chromatography based on the ionic capacity
Thiemo Huuk, Tobias Hahn, Jürgen Hubbuch
Biotechnology Progress 05/2016; 32(3):666–677. DOI:10.1002/btpr.2228

Application of spectral deconvolution and inverse mechanistic modelling as a tool for root cause investigation in protein chromatography
Nina Brestrich, Tobias Hahn, Jürgen Hubbuch
Journal of Chromatography A 03/2016; 1437:158–167. DOI:10.1016/j.chroma.2016.02.011

Modeling and simulation of anion-exchange membrane chromatography for purification of Sf9 insect cell-derived virus-like particles
Christopher Ladd Effio, Tobias Hahn, Julia Seiler, Stefan Oelmeier, Iris Asen, Christine Silberer, Louis Villain, Jürgen Hubbuch
Journal of Chromatography A 01/2016; 1429:142-154. DOI:10.1016/j.chroma.2015.12.006

Insights into chromatographic separation using core-shell metal-organic frameworks: size exclusion and polarity effects
Weiwei Qin, Martin E. Silvestre, Frank Kirschhöfer, Gerald Brenner-Weiss, Matthias Franzreb
Journal of Chromatography A 09/2015; 1411:77–83. DOI:10.1016/j.chroma.2015.07.120

Simulating and Optimizing Preparative Protein Chromatography with ChromX
Tobias Hahn, Thiemo Huuk, Vincent Heuveline, Jürgen Hubbuch
Journal of Chemical Education 09/2015; 92:1497–1502. DOI:10.1021/ed500854a

High-throughput micro-scale cultivations and chromatography modeling: Powerful tools for integrated process development
Pascal Baumann, Tobias Hahn, Jürgen Hubbuch
Biotechnology and Bioengineering 10/2015; 112:2123–2133. DOI:10.1002/bit.25630

Calibration-free inverse modeling of ion-exchange chromatography in industrial antibody purification
Tobias Hahn, Thiemo Huuk, Anna Osberghaus, Katharina Doninger, Susanne Nath, Stefan Hepbildikler, Vincent Heuveline, Jürgen Hubbuch
Engineering in Life Sciences 05/2015; 16:107–113. DOI:10.1002/elsc.201400248

UV absorption-based inverse modelling of protein chromatography
Tobias Hahn, Pascal Baumann, Thiemo Huuk, Vincent Heuveline, Jürgen Hubbuch
Engineering in Life Sciences 04/2015; 16:99–106. DOI:10.1002/elsc.201400247

Model-based integrated optimization and evaluation of a multi-step ion exchange chromatography
Thiemo C. Huuk, Tobias Hahn, Anna Osberghaus, Jürgen Hubbuch
Separation and Purification Technology 11/2014; 136:207–222. DOI:10.1016/j.seppur.2014.09.012

Adjoint-based Estimation and Optimization for Column Liquid Chromatography Models
Tobias Hahn, Anja Sommer, Anna Osberghaus, Vincent Heuveline, Jürgen Hubbuch
Computers & Chemical Engineering 05/2014; 64. DOI:10.1016/j.compchemeng.2014.01.013

A high-throughput 2D-analytical technique to obtain single protein parameters from complex cell lysates for in silico process development of ion exchange chromatography
Frieder Kröner, Dennis Elsäßer, Jürgen Hubbuch
Journal of Chromatography A 11/2013; 1318. DOI:10.1016/j.chroma.2013.09.043
ChromX – A Powerful and User‐Friendly Software Package for Modeling Liquid Chromatography Processes
Tobias Hahn, Vincent Heuveline, Jürgen Hubbuch
Chemie Ingenieur Technik 08/2012; 84(8). DOI:10.1002/cite.201250071