Gain a Mechanistic Understanding of Your Processes!
All processes happening within the column are usually hidden from the operator. Only the total UV absorption of all species at the outlet of the column can be recorded. The challenge of chromatography modeling lies in describing convection, diffusion, competitive adsorption and reactions mechanistically.
The video shows a step elution on a cation-exchange column. The plot in the upper right corner shows the buffer salt concentration at inlet and outlet, the lower right plot shows the protein pulse injection and chromatogram. The 3D plots visualize what is happening in the fluid outside the particles (left), inside the pores (center) and in the stationary phase (right).