Glowing light bulb on a blue background

Process understanding

Generated by mechanistic models

scroll for more

Process understanding empowered by mechanistic models

Chromatography is a complex superposition of different effects: fluid dynamics, mass transfer phenomena and thermodynamics of phase equilibria. Mechanistic models include all of these effects, denoted in terms of the mathematical language. Many process parameters are directly included in the model equations, and process quality attributes can be calculated from the simulation results. In this way, parameter studies allow the examination of positive or negative effects on the process before they occur in production.

Click on the button to load the content from .

Load content

Snapshot of video on mechanistic models
YouTube

By loading the video, you agree to YouTube's privacy policy.
Learn more

Load video

Chromatography simulation enables intra-column understanding

The result of a chromatography simulation is much more than just a plain chromatogram. Mechanistic models capture the movement of individual molecules along their journey from the interstitial volume of the mobile phase to the pore volume of the mobile phase and finally to the volume of the stationary phase.

Liquid phases in a chromatography column
From left to right: interstitial volume of the mobile phase, pore volume of the mobile phase and volume of the stationary phase.

All of these three volumes are explicitly considered in a simulation and species concentrations are continuously calculated at every location and every point in time.

In a real experiment, you typically only get the pool fractions at the column outlet. In a simulation, you can access species concentrations everywhere in the column and at every time. In this way, you earn valuable intra-column process understanding.

Flow rate induced convection

The injected molecules are transported with the fluid flow which is induced by a connected pump. The effect of convection is foremost visible via a shifted peak in time. Pumping with higher velocity leads to higher convection. This can be seen in an earlier peak, where the flowrate defines the position of the peak. Plotted over volume, the peak position and shape might change as well. An increased velocity will lead to less peak broadening and will also affect mass transfer processes. Apart from the pump speed, a second cause for change in flow rate can be varying packing quality. Mechanistic models consider the porosity of the chromatography column as a model parameter which influences the linear flow rate inside the column. The denser the packing, the higher the linear flow.Depiction of convection in mechanistic modelling

Column packing quality and dispersion

Dispersion comprises different flow effects outside the particle’s pores, such as flow distributors, wall effects, turbulence, eddy diffusion and molecular diffusion. These random diffusive movements and non-ideal flow patterns cause peak broadening. Compared to the convectional effects, dispersive forces have a greater influence on the shape of the chromatogram. Dispersion is a time-dependent process. The longer molecules stay inside the column, the longer they are affected by dispersion. When assessing column packing quality with a tracer pulse, a slight tailing is a natural result of the peak front being less affected by dispersion in comparison to the back slope which needs longer to pass the column.Depiction of dispersion in mechanistic modellingsion

Flow rate dependent pore accessibility and slow pore diffusion

Given that the pore is accessible, a molecule can pass a particle’s so-called stagnant fluid film and enter the particle’s pore volume. The resulting transition between interstitial volume and pore volume is also denoted as a mass transfer from one volume to the other. This transfer can also be velocity-dependent. In the case of a high flow rate, the likelihood of a molecule passing a bead and not entering its pore system is higher. Once a component has entered a particle’s pore, its movement is dominated mainly by diffusion. Both pore accessibility and slow pore diffusion lead to asymmetric or tailed peaks.Depiction of diffusion in mechanistic modelling

Raw material and upstream variability influencing adsorption

Finally, adsorption onto free binding sites, interfered with by the previous effects, leads to the final shape of your chromatogram. Large molecules such as antibodies usually bind to several ligands at once. Most chromatography models capture this behavior by considering multi-point binding. The concentration of ligands is described with the help of the resin capacity that is provided by most manufacturers.

Competition for free binding sites plays another integral part of chromatography and is captured by chromatography models. Competitive adsorption naturally depends on the individual concentrations of all species. A change in upstream conditions leading to a different feed stock composition can be simulated directly without a recalibration of the model. The same holds true for a change in binding and elution conditions by varying, for example, salt concentrations and pH in silico.Depiction of adsorption in mechanistic modelling

Do NOT follow this link or you will be banned from the site!